认识一下sacB gene - 知乎专栏
Aug 16, 2023 ... 还有人像我一样不认识sacB基因的功能和应用吗SacB基因在大肠杆菌中表达蔗糖:2,6-对-D-果聚糖6-3-D-果糖基转移酶,在培养基中含有5%蔗糖的条件下, ...
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Aug 16, 2023 ... 还有人像我一样不认识sacB基因的功能和应用吗SacB基因在大肠杆菌中表达蔗糖:2,6-对-D-果聚糖6-3-D-果糖基转移酶,在培养基中含有5%蔗糖的条件下, ...
SacB基因是来自枯草芽孢杆菌的糖代谢相关基因,其作用为将蔗糖转变为果聚糖。由于大肠感觉缺乏处理果聚糖的基因,在大肠杆菌中表达SacB基因,会导致SacB基因的产物果聚糖在 ...
枯草芽孢杆菌(Bacillus subtilis)sacB基因编码的果聚糖蔗糖酶,是枯草芽孢杆菌的一种外分泌酶,它受蔗糖诱导表达,能将蔗糖分解成葡萄糖和果糖,还可进一步将果糖聚合成果聚糖 ...
The structural gene sacB encoding B. subtilis levansucrase, a secreted enzyme, expresses in E. coli. E. coli hosts of the sacB gene are poisoned by sucrose.
Jul 4, 2024 ... 图1 PCR扩增Amp启动子及sacB基因及融合片段. M:分子量2 000 bp的Marker;1:Amp启动子(PAmp. )片段;. 2:sacB基因片段;3:Amp启动子与sacB基因的融合PCR产物。
通过测定蔗糖水解产生的葡萄糖具有的还原性,确定sacB基因产物具有蔗糖果聚糖酶活性。该基因表达后,导致大肠杆菌(Escherichia coli)细胞不能在含5%蔗糖的培养基中生存。同时 ...
Aug 6, 2025 ... In the present study, we developed a SacB-based system, pK18mobsacB, to achieve marker-free gene editing with an efficiency of up to 83.33%.
表达SacB基因的载体构建成功之后(已测序验证),将含有该载体的大肠杆菌DH5α涂布至含有10%蔗糖的LB平板,发现可以正常生长,文献报道含有SacB基因的大肠杆菌在蔗糖浓度为5% ...
P05655 · LSC_BACSU. Protein. Levansucrase. Gene. sacB. Status. UniProtKB reviewed (Swiss-Prot). Organism. Bacillus subtilis (strain 168). Amino acids. 473 (go ...
SacB-SacR genes can be used as negative selection markers to suppress the overgrowth of A. tumefaciens in the plant tissue culture process.