URA3基因_百度百科
URA3基因是酵母菌V号染色体上的编码基因,其产物乳清苷5-磷酸脱羧酶参与尿嘧啶核苷酸的生物合成。 该基因在基因编辑技术中被广泛用作筛选标记,通过5-氟乳清酸(5-FOA)毒性筛选系统实现转化菌株的高效筛选。
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URA3基因是酵母菌V号染色体上的编码基因,其产物乳清苷5-磷酸脱羧酶参与尿嘧啶核苷酸的生物合成。 该基因在基因编辑技术中被广泛用作筛选标记,通过5-氟乳清酸(5-FOA)毒性筛选系统实现转化菌株的高效筛选。
Nov 17, 2005 · URA3 encodes orotidine-5'-phosphate decarboxylase, an enzyme involved in pyrimidine biosynthesis. It is also a marker gene for uracil auxotrophy and resistance to 5-FOA. Learn more about its sequence, a...
URA3 ... URA3 是 釀酒酵母 (Saccharomyces cerevisiae)V号 染色体 上的一个 基因,系统名称为 YEL021W。 URA3 编码 乳清酸核苷-5'-磷酸脱羧酶 (ODCase),催化 嘧啶 生物合成的关键一步反应,常在 酵母 的研究中作为一种“标记基因”使用 [1]。
URA3, Kl - URA3 is defined as a genetic marker used in yeast genetics that allows for the generation of isogenic strains with targeted mutations, facilitating the study of gene structure and function, particularly in ...
The mutation of the selection marker URA3 was supposed to affect the growth and artemisinic acid production. A ura3 mutant was successfully restored by a recombinant plasmid pML104-KanMx4-u along with a 90 bp donor DN...
URA3 ... URA3 is a gene on chromosome V in Saccharomyces cerevisiae (yeast). Its systematic name is YEL021W. URA3 is often used in yeast research as a "marker gene", that is, a gene to label chromosomes or plasmids.
Feb 8, 2025 · We found that mutations were scattered throughout the open reading frame of the URA3gene but clustered to several hotspots with base substitutions/deletions at homo-polymeric runs. Surprisingly, we only ...
当酵母细胞中含有URA3基因时,细胞能够合成足量UMP来对抗5-FOA代谢产物5-FU的毒性,从而保证细胞存活;而当酵母细胞中不含URA3基因时,则无法合成足量UMP来对抗5-FU的毒性,最终导致细胞死亡。 三、应用 URA3筛选标记广泛应用于酵母遗传学中。
中文摘要: 构建了营养缺陷型解脂耶氏酵母菌株,使之用于遗传标记和高产香味物质γ-癸内酯。 作者利用基因同源重组的方法敲除掉尿嘧啶合成酶关键基因URA3基因,用尿嘧啶营养缺陷型培养基 (SD-URA)添加一定浓度的5-氟乳清酸 (5-FOA)和尿嘧啶筛选获得转化子。
摘要: 【背景】 表达载体是基因工程必不可少的工具,对于真菌如里氏木霉 (Trichodermareesei)等,由于缺乏商品化的表达载体,使其基因工程蛋白表达既复杂又费时而难以开展。